Abstract

QUINTERO MORA, Leidy Patricia et al. Evaluation of ethanol production from potato, cassava and orange wastage in discontinuos cultivation using Saccharomyces cerevisiae. Rev. ion [online]. 2015, vol.28, n.1, pp.43-53. ISSN 0120-100X.

The present research aimed to produce ethanol using glucose syrup made from potato, orange and cassava wastage, using discontinuos cultivation as an alternative to handle the wastage generated. In order to meet the main objective two strains of Saccharomyces cerevisiae were used, a native strain (LYP) and control strain (CTL) obtained from the strain stock of the University. Subsequently, for the preparation of the fermentation mediums, the wastage was subjected to a chemical hydrolysis treatment with hydrochloric acid 2%v, at a temperature of 75°C for 8 hours, these parameters were selected as a result of statistical analysis of the factorial design 3³ which throws statistically significant differences (p≥0.05) in the variables evaluated on the generation of reducing sugars. Finally, 500ml fermentations were performed at 30°C, 150rpm based in a factorial design 4² in order to evaluate the fermentative substrates and fermenting microorganism, where no significant differences were found (p≥0.05), for both substrates and strains: (CTL) and (LYP), in ethanol production. However, regarding the interactions between the variables, some highly significant differences were found (p≤0.05), the highest production of ethanol obtained was 3.15 and 2.6% for strains LYP and CLT respectively, when they used the orange syrup and cassava as carbon source.

Keywords : chemical Hydrolysis; ethanol; waste.

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