Resumo

OSCANOA HUAYNATE, Alberto I; YNGA HUAMAN, Gheraldine A; CHANG AVILA, Iliana L  e  AGUILAR SAMANAMUD, Carla P. Impact of CO₂ on cell density in six marine microalgae strains. Rev. ion [online]. 2015, vol.28, n.2, pp.23-32. ISSN 0120-100X.  https://doi.org/10.18273/revion.v28n2-2015002.

As microalgae can capture CO₂ easily from the environment, it is interesting to measure the amount and time control of the entry of this gas into microalgae mass culture, in order to increase cell density. The aim of this study was to evaluate the injection times of CO₂ for biomass production that may lead to a higher cell density, it was also evaluated the pH variation without altering the quality of the crop. The work was made with six strains from the Germplasm Bank of the Instituto del Mar del Perú. There were performed cultures like 300L batch in a greenhouse, the cultivation time of the exponential phase lasted three days. The slope of the regression line parameters was analyzed to process data. The results showed that the cell density is inversely proportional to CO₂ injection time cultivation. The higher cell density was obtained after 5min in different strains, except for strains Chaetoceros gracilis and Nannochloris maculate. Those microalgae got the highest density at 10 and 15min, respectively. The variation of pH tended toward acidity in a range from 8 to 4, without altering the cell density and the cultures remained free of contaminants. In conclusion, the results can establish the appropriate times of CO₂ injection, which strengthen the exponential growth phase by increasing population density in 30% over the usual results of this phase

Palavras-chave : microalgae; cell density; CO; massive culture.

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