Abstract

PEREZ-CABEZA, Susan B.; MORON ANGARITA, Nicolás; CERVANTES, Martha  and  BARON-RODRIGUEZ, Mario A.. Evaluation of antioxidant potential in a spinach extract by cyclic voltammetry. Rev. ion [online]. 2017, vol.30, n.2, pp.99-105. ISSN 0120-100X.  https://doi.org/10.18273/revion.v30n2-2017009.

The antioxidant capacity of plant extracts has been studied by voltammetric techniques thanks to its sensitivity, selectivity, low cost, simplicity and speed. In this research, Cyclic Voltamperometry was used to study the antioxidant potential and stability in ethanolic extracts of spinach leaves (Spinacia oleracea) over time. The extracts were obtained after ultrasound of macerating spinach leaves with industrial ethanol. The potentiometric sweeps of the extracts at 0, 7 and 15 days of preparation were carried out in a Sørensen buffer. Electrodes of Au (work), Ag/AgCl (reference) and Pt (auxiliary) were used from -1.2 to 1.2V. The results showed a high antioxidant capacity of the extracts (oxidation potentials below 600mV) and a decrease in the current while the extracts preservation (0, 7 and 15 days), which allows the conclusion that its Antioxidant potential decreases over time describing a first-order kinetic. Also, the results of the antioxidant capacity were correlated with the Folin Ciocalteu method.

Keywords : free radicals; oxidative stress; cyclic voltammetry; spinach extract; antioxidant potential.

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