Abstract

TORRES, Golda Meyer; SARMIENTO, Olga Isabel; RAMIREZ, Ruth Isabel  and  GUEVARA, Osvaldo. Estimation of the content of total phenols in calendula essential oil (Calendula Officinalis L) obtained by OAHD. Rev. ion [online]. 2018, vol.31, n.1, pp.7-12. ISSN 0120-100X.  http://dx.doi.org/10.18273/revion.v31n1-2018001.

This work forms part of the preliminary characterization of calendula (Calendula officinalis L.) essential oil obtained by two methods: hydrodistillation (HD) and ohmic-assisted hydrodistillation (OAHD); fresh flowers (Ff) and dried flowers (Fs) were used. Total phenols content was estimated with Folin-Ciocalteu reagent using tannic acid as standard (μg /mL). The effect of the methods and treatments on the total phenols content (μg/mL) was evaluated; results obtained showed significant differences (p <0.05) when comparing the interaction between flower states for the same extraction method, but this was not the case when flower states were compared irrespective of the extraction method (p≥ 0.05); therefore, the extraction methods used did not show effects on the content of phenols in each of the oil samples while the drying process does have an impact on the development of phenolic structures in the oil samples. It is necessary to continue working on the characterization of calendula flower essential oil obtained by HD and OAHD with regard to the chemical composition of each of the fractions as well as the measurement of the antioxidant activity using higher sensitivity analytical techniques.

Keywords : quantification of phenols and flavonoids; content of total phenols; calendula essential oil; hydrodistillation of essential oils; ohmic heating.

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