Resumo

MURILLO-USUGA, Carlos Augusto  e  ESCOBAR-SIERRA, Diana Marcela. Optimization of the fibroin extraction process from the Bombyx Mori silkworm cocoon. Rev. ion [online]. 2022, vol.35, n.1, pp.33-42.  Epub 03-Jun-2022. ISSN 0120-100X.  https://doi.org/10.18273/revion.v35n1-2022003.

In the present work, a statistical study was carried out to optimize the performance of the fibroin extraction process from the cocoon of the silkworm Bombyx Mori, also known as degumming, in which fibroin and sericin, components that make up the cocoon structure, are separated using aqueous solutions of sodium carbonate (Na CO ); the study was done through an experimental design 23 with two central 23 points. For this purpose, the selected parameters were temperature, the Na2CO3/Cocoons ratio and extraction time. The statistical significance of these factors was studied by analysis of variance (ANOVA). According to the results, the extraction process depends mainly on the extraction time (p = 0.009) and the working temperature (p = 0.0112), obtaining an average of 74.76% fibroin from the cocoon under optimal extraction conditions. Finally, the sample obtained under the best conditions was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and by Thermo Gravimetric Analysis (TGA) in order to have the basis of the material obtained for possible biomedical applications.

Palavras-chave : Fibroin; Degumming; Optimization.

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