Abstract

ARDILA ARIAS, Alba Nelly et al. Validation of a high-performance liquid chromatographic method for the simultaneous determination of diclofenac and ibuprofen in water resources. Rev. ion [online]. 2022, vol.35, n.2, pp.111-125.  Epub Apr 25, 2023. ISSN 0120-100X.  https://doi.org/10.18273/revion.v35n2-2022009.

Diclofenac sodium and ibuprofen are highly consumed drugs, used for the relief of common ailments, their poor metabolization means that a large proportion of them become aquifer contaminants, this problem requires urgent attention. The monitoring of these contaminants, through the development and validation of analytical methodologies that allow their quantification in water resources, becomes a necessity to prevent and control them. In this study, a high-performance liquid chromatography method was developed for the simultaneous determination of diclofenac and ibuprofen in water. The validation of the method was carried out with the determination of linearity, limits of quantification and detection, accuracy and precision in terms of repeatability and intermediate precision. For the determination of the performance parameters, the percentage recovery, average, standard deviation, relative standard deviation, coefficient of variation and confidence interval were calculated. In addition, to determine the accuracy and precision, the factorial analysis of variance was applied with a confidence level of 95 % and a significance level of 0.05. The values obtained for all parameters complied with established acceptance criteria, confirming that the method developed is accurate and precise with high levels of reliability.

Keywords : Method validation; High-performance liquid chromatography; Diclofenac; Ibuprofen; Water resources.

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