Abstract

ACUNA MONSALVE, Yudtanduly; CASAS FORERO, Nidia; BENAVIDES TORRES, Matía Isabel  and  SIERRA GARCIA, Manuel Eduardo. Use of microalgae residual biomass to obtain protein hydrolysates as input in the production of aquaculture feed. Rev. ion [online]. 2024, vol.37, n.3, pp.7-13.  Epub Nov 28, 2024. ISSN 0120-100X.  https://doi.org/10.18273/revion.v37n3-2024001.

With the purpose of adding value to the residual biomass produced after the oil extraction process from microalgae, a methodology was developed to obtain a protein hydrolysate as a source of essential amino acids that could be used as an ingredient in the production of aquaculture feed. The methodology includes stages of microalgae cultivation, chlorophyll extraction, protein extraction and protein hydrolysis.

Eight duplicate tests were obtained in protein extraction, in which the best yield was 72.96 °% at 45 °C and pH 13 for 120 minutes. Likewise, in the protein hydrolyzate, 6 duplicate tests were performed using the enzyme pepsin, where the highest degree of hydrolysis (21.98 %) was obtained at pH 3 and 37 °C, with an enzyme substrate ratio of 2 %w for 4 hours. According to the processes with the highest yield, characterization and evaluation were carried out, from which it was concluded that the methodology described to obtain the protein hydrolysate was successful because of the presence of essential amino acids for aquaculture feed.

Keywords : Hydrolyzate; Protein; Amino acids; Aquaculture.

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