Abstract

VEGA, Miguel Ángel  and  CASTILLO, John J.. Sweet potato peel peroxidase-modified graphene oxide electrodes for detection of hydrogen peroxide via electrochemical sensing. Rev. ion [online]. 2024, vol.37, n.3, pp.43-54.  Epub Dec 09, 2024. ISSN 0120-100X.  https://doi.org/10.18273/revion.v37n3-2024004.

The development of efficient and sensitive detection methods for hydrogen peroxide (H2O2) is crucial for various applications in biology, medicine, and environmental monitoring. Here, we present a novel approach utilizing sweet potato peel peroxidase (BPP) extract-modified screen-printed graphene oxide electrodes (SPGOE) for the electrochemical sensing of H2O2. The SPP was characterized as having a specific activity of 478 U mg-1, an optimum pH of 8.0 and a thermostability at 60 °C with a Kinact of 7.02x10-3 min-1. In this study, we systematically investigate the fabrication process of the peroxidase batata-modified SPGOE and characterize their electrochemical performance using cyclic voltammetry technique. Scanning electron microscopy (SEM) was used to analyze the surface morphology of the modified electrodes, revealing successful enzyme immobilization with a marked increase in surface roughness and visible enzyme clusters compared to the bare SPGOE. This surface modification supports efficient electron transfer, which contributes to the sensor's enhanced electrocatalytic performance. The SPP-SPGOE demonstrates outstanding electrocatalytic performance for the reduction of H2O2, showing a linear response across the 250 μM to 5 mM concentration range and a detection limit of 4.6 mM. This novel sensor, created by incorporating SPP onto the GO electrode, offers a promising electrochemical detection system for measuring H2O2 in real-world samples, which has significant biomedical and environmental applications. Overall, this study presents a versatile and efficient strategy for electrochemical sensing of H2O2 using SPP-SPGOE, paving the way for advanced analytical methodologies with broad applications in biology and beyond.

Keywords : Sweet potato peel; Peroxidase; Cyclic voltammetry; Sensing; Hydrogen peroxide.

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