Resumo

GOMEZ GARCIA, Liliana  e  MARTINEZ, Sixta Tulia. INDUCTION OF TWO PECTOLITIC ENZYMES DURING THE MODEL Fusarium oxysporum f. sp. dianthi - CARNATION. Rev.Colomb.Quim. [online]. 2005, vol.34, n.1, pp.25-34. ISSN 0120-2804.

The Polygalacturonase (PG; EC 3.2.1.15) and the Pectate lyase (PL; EC 4.2.2.2) are considered as factor of virulence. These enzymes were studied during the infection process of Fusarium oxysporum f. sp. dianthi (FOD) on Carnation (Dianthus caryophillus L.) using culture medium in vitro. The results show the induction of the expression of the PG enzyme when the artificial inductors polygalacturonic acid (APG) and pectin are present. The level of expression was very low in culture with cellular wall (PC) of carnations of the resistant and susceptible varieties. The PL enzyme did not show expression in the presence of artificial inductors (APG and pectin) but in induced culture with root and cellular wall showed a high level of expression.

Palavras-chave : Polygalacturonase; pectate lyase; pectin enzymes; Fusarium oxysporum f. sp. dianthi.

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