Resumo

DALLOS CORREDOR, David  e  GUERRERO DALLOS, Jairo Arturo. METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF PESTICIDES IN GREEN COFFEE BY GAS CHROMATOGRAPHY. Rev.Colomb.Quim. [online]. 2005, vol.34, n.2, pp.175-188. ISSN 0120-2804.

This study describes the implementation and validation of a multiresidue methodology for the determination of organochlorine, organophosphorus and pyrethroids pesticides in green coffee. Pesticides residues were extracted from green samples with an acetone-water (2:1) mixture followed by ethyl acetate-cyclohexane (1:1) partitioning. The clean up steps include gel permeation chromatography and mini column chromatography using silicagel. Final determination was carried out by high resolution gas chromatography with a pulsed splitless injection mode and simultaneous detection by µ-ECD and NPD coupled in parallel. The methodology is specific, selective precise and accurate. Recoveries of majority of pesticides from spiked samples range from 70 to 110% at fortification levels of 0.038 mg/kg-1.536 mg/kg with limit of quantitation between 0.011 mg/kg and 0.100 mg/kg.

Palavras-chave : pesticides; coffee; gas chromatography; clean up; validation.

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