Resumo

PINZON, Martha L; ORTIZ, Blanca L  e  SANCHEZ DE GOMEZ, Myriam Y. RELATIONSHIP BETWEEN METALLOPROTEINASES 2 AND 9 AND TIMP-1 INHIBITOR mRNA EXPRESSION IN HYDATIDIFORME MOLE . Rev.Colomb.Quim. [online]. 2007, vol.36, n.2, pp.137-149. ISSN 0120-2804.

At the beginning of gestation the Insulin Like Growth Factor II (IGF-II) is highly expressed in placenta and it could regulate the invasive behavior of trophoblastic cells. This invasive ability depends on its capability to secret metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs), abnormal expression of which could contribute to several pathological processes.In the Gestational Trophoblastic Diseases (GTD) these factors could be unregulated, in this way complete hydatidiforme mole (CHM) produces the choriocarcinoma which is an invasive and metastasic tumor. The aim of this study was to investigate the relationship between IGF-II, MMP-2, MMP-9 and TIMP-1 mRNA levels by RT-PCR technique, in hydatidiform mole tissues vs. first normal trimester placentas. MMPs activity was evaluated using zimography assay finding that in CHM this is increased, which could relate trophoblast malignization with an increased invasive capability. The higher IGF-II mRNA expression in this pathology could, also, be associated with increased MMPs activity. A higher MMP-9/ TIMP-1 mRNA expression ratio in CHM is suggested as a predictor of trophoblastic tissue malignization .

Palavras-chave : metalloproteinases; Type II Insulin Like Growth Factor; Complete Hydatidiform Mole.

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