Revista Colombiana de Química
Print version ISSN 0120-2804
SERNA-COCK, Liliana; PINEDA-CUERVO, Darío and AYALA-APONTE, Alfredo. APPLICATION OF ALKALINE ENDOPEPTIDASE IN BOVINE HIDE TO IMPROVE THE QUALITY OF THE GELATINE . Rev.Colomb.Quim. [online]. 2007, vol.36, n.2, pp. 169-181. ISSN 0120-2804.
The international market price of gelatine is determined by its physical-chemical properties, that depend on the raw material, the collagen extraction method and the concentration method. In this work, using class B gelatine production processes in pilot plant, the effect of application of alkaline endopeptidase was evaluated in the pre-treatment of three types of bovine hide on the gelatine final quality as measured by bloom, viscosity and clarity. The results were compared with processes in which the same raw materials were used without enzymatic pre-treatment. The statistical analysis showed significant differences among the treatments. The best bloom, viscosity and clarity results were presented using enzymatic pre- treatment in the whole hide, obtained in thirteen hours of process 61.5% of the gelatine with bloom higher than 300g, 51.3% with viscosities values higher than 42 mp and 82 ,1 % with high clarities value, with values lower than 39 nephelometric turbidity units. The whole hide without enzymatic pre-treatment showed 41% of high values of bloom, 7.7% of high viscosities and 76.9% of high clarities .
Keywords : gelatina; protease; bovine hide.