Abstract

FORERO R, Francy L; ORTIZ Q, Blanca L  and  ANZOLA V, Cecilia. EVALUATION OF THE MODULATING EFFECT OF TYPE-I INSULIN-LIKE GROWTH FACTOR (IGF-I) IN RAT SPLEEN-T LYMPHOCYTES UNDER NUTRITIONAL STRESS. Rev.Colomb.Quim. [online]. 2008, vol.37, n.3, pp.305-316. ISSN 0120-2804.

In this work we studied the role of Insulin- like Growth Factor-I (IGF-I) as stress modulator in spleen T-lymphocytes from protein restricted rats. In protein restriction we found 15% lowered body weight, 32% lowered spleen weight and a reduction of 42% in total T-cell population. Also, increased percentages of T-CD4, T-CD8 and B cell populations and lowered T-CD4/T-CD8 ratio was observed, suggesting an impaired immune function due to malnutrition. Cultures of spleen T-lymphocytes from protein restricted rats showed less proliferation than the ones from well fed rats, this proliferation was increased after the addition of IGF-I in a dose depending manner. This answer depends, also, from the protein content of the diet, since the well fed animals showed a higher and faster answer. Concanavaline A, also, increased proliferation in both groups of rats and when combined with IGF-I a synergistic answer was found. In this work we also found that protein restriction increased apoptosis of T-lymphocytes, and the addition of IGF-I helped in the recovery of those cells supporting the immunomodulator role of IGF-I in nutritional stress.

Keywords : Type I Insulin-like Growth Factor (IGF-I); lymphocytes; protein restriction; apoptosis; immunomodulator.

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