Revista Colombiana de Química
versión impresa ISSN 0120-2804
MARTIN, Jenny C. y LOPEZ, Elizabeth. PHYSICAL MODIFICATION OF CASSAVA STARCH AND EVALUATION OF SUSCEPTIBILITY TO ENZYME HYDROLYSIS BY ALPHA AMYLASE. Rev.Colomb.Quim. [online]. 2009, vol.38, n.3, pp. 395-408. ISSN 0120-2804.
Cassava starch (MTAI 8 variety) was subjected to syneresis, gelatinization, extrusion and processing by drum dryers. The morphology, crystallinity, molecular weight distribution and susceptibility to enzyme hydrolysis by porcine pancreatic a-amylase were determined before and after the physical treatments. The physically modified starches increased the extent of a-amylolysis, compared to the native one. However, there were not significant differences in the degree of amylolisis between the treatments during the procedure. Both the X-ray pattern type-A, presentedinthecassavastarch, and its birrefringent property, observed in polarized light, were altered by the treatments causing amorphous structures and the loss of the Maltese cross. When the modified starches were observed n scann ng electron micrographs (SEM), an alteration in the appearance and structure of the native granule was shown, where particles with irregular shape and fragmented and wrinkled surfaces could be seen as a result of the modification process. The profile of the size exclusion chromatography on sepharose 6B showed two characteristic fractions of high and low molecular weight in the native starch, while in modified starches only one peak was obtained showing low molecular weight. The data showed that the treatments modified the physical structure of the starch granule, allowing more accessibility for the enzyme to the amorphous and crystalline regions of starch.
Palabras llave : Cassava starch; syneresis; gelatinization; extrusion; drum dryers; enzyme hydrolysis; a-amylase.