Abstract

REYES-MONTANO, Edgar A; LAREO, Leonardo R  and  PEREZ, Gerardo. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTOR. Rev.Colomb.Quim. [online]. 2012, vol.41, n.1, pp.31-45. ISSN 0120-2804.

Producing polyclonal antibodies (IgY) in chickens has advantages over those obtained in other animal models, since they have been used as a tool for studying different proteins (NMDA glutamate receptor in our case, specifically the NR1 subunit). We produced specific antibodies against expression products by the alternative splicing of the gene encoding NMDA receptor NR1 subunit in adult rat brain. Three peptides corresponding to the splicing sites (N1, C1 and C2' cassettes) were designed, synthesised and used individually as antigens in hens. Specific immunoglobulins were purified from yolks. The antibodies were then used for purifying the NMDA receptor NR1 subunit using affinity chromatography coupling the three antibodies to the support.

Keywords : IgY; antibody; glutamate; NMDA; NR1 subunit; affinity chromatography; purification.

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