Abstract

ALBARRACIN C, Sonia Luz; RODRIGUEZ L, Edwin A; PEREZ G, Gerardo  and  REYES, Edgar Antonio. INTERACCIÓN DE LA GLUTAMINA SINTETASA (GS) Y EL PÉPTIDO β -AMILOIDE COMO UNA ESTRATEGIA DE PURIFICACIÓN [title language="en"]INTERACTION OF GLUTAMINE SYNTHETASE (GS) AND AMYLOID β-PEPTIDE AS A PURIFICATION STRATEGY. Rev.Colomb.Quim. [online]. 2012, vol.41, n.2, pp.179-194. ISSN 0120-2804.

Alzheimer's disease (AD) is the most common form of dementia in adulthood; it is manifested by the progressive loss of memory since neurons in both cerebral cortex and hippocampus die. In all the forms of AD is observed the increased expression of different proteins, as well as the presence of insoluble aggregates of β-amyloid peptide (BAP). Glutamine synthetase (GS) is a key enzyme in the metabolism of glutamate and in the detoxification of ammonium (NH4⁺). A possible interaction GS-PBA has been previously reported and it can be associated with AD. In this work we performed the purification of the enzyme from rat brain extract subjected to fractional precipitation 20-60 % saturation with (NH₄)₂SO₄, and thereafter through successive chromatographies of gel filtration, ion exchange and affinity. The molecular weight of the complex was calculated at 137 kDa by the order of elution in the column filtration. The interaction of the enzyme with 1-40 PBA was identified, achieving the purification of a single band of 45 kDa corresponding to the monomeric form of the GS. In this paper we present a new method of the enzyme purification and we demonstrated the interaction of GS with the PBA. We propose this interaction GS-PBA can be one of the processes that occur in the disease and it could explain the reduction in enzyme activity in patients with AD, since it might alter the glutamate-glutamine cycle and generate changes in the cellular environment which favor glutamate excitotoxicity typical of neurodegeneration processes.

Keywords : Glutamine synthetase; protein purification; beta amyloid peptide.

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