Resumo

AHUMADA F, Diego A; RODRIGUEZ L, Danny; ZAMUDIO S, Adriana  e  MOJICA C, Andrea. COMPARISON OF DIFFERENT APPROACHES TO ESTIMATE LIMITS OF DETECTION FOR PESTICIDE RESIDUES ANALYSIS IN FOOD. Rev.Colomb.Quim. [online]. 2012, vol.41, n.2, pp.227-242. ISSN 0120-2804.

The limit of detection (LOD) calculation is a practice that has generated several discussions in the scientific community, due to different definitions and procedures for its estimation. The fundamental objective of the present work is to apply, confirm and compare six different recommendations suggested by official guidelines for the estimation of LOD of 31 pesticide residues in five different commodities using the QuEChERS method and ultra fast liquid chromatography coupled to mass spectrometry. The methods evaluated were IUPAC, EPA, t99, root mean square error (RMSE), Hubaux- Vos and propagation of errors. In this study it was found that limits of detection of the different approaches did not show a significant variation with the commodities tested, excluding the method of EPA. On the other hand, t99, RMSE and Hubaux methods showed the lowest signal to noise ratio (S/N<3). Furthermore, the EPA approach showed the highest S/N, while IUPAC and propagation of errors methods presented the best S/N. Finally, no method estimated the limits of detection for all compounds with ratios S /N appropriate.

Palavras-chave : pesticides; QuEChERS; limit of detection; food.

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