Abstract

TORRES L, Gina A; SANCHEZ B, Iván C; RESTREPO S, Luz P  and  ALBARRACIN H, William. STUDY OF LAMB MEAT AGEING USING ELECTROPHORESIS SDS-PAGE. Rev.Colomb.Quim. [online]. 2012, vol.41, n.2, pp.263-282. ISSN 0120-2804.

The ageing of meat is caused by metabolic reactions than result in changes in its quality, improving its organoleptics characteristics such as the tenderness, the juiciness and the color. This study had the aim to find changes in sarcoplasmic and myofibrillar proteins during the establishment of rigor mortis and the ageing in lamb meat: semi feedloot (SF) and lamb feeding for pasture (FP). For this purpose, were sampled Longissimus dorsi muscle of lambs bred Dorper x Criollo ¾ and 10 weeks old. Sarcoplasmic proteins were extracted with water or saline solution diluted and the myofibrillar protein with saline solution concentrate. The Quantification was done by the Bradford method and the separation by SDS-PAGE electrophoresis. In the sarcoplasmic proteins observed the appearance of a band of 146 kDa in the samples of five and thirteen days for lamb SB and AP, respectively. In the myofibrillar proteins found evidence of the establishment of rigor mortis at 16 hours and a drastic intensification of a band of 27 kDa to 16 hours in the lamb SB. This band was also observed in the lamb FP at 14 hours. In the whole process could see the gradual degradation of Desmin, Actin and Troponin T.

Keywords : Meat; ageing; electrophoresis; lamb.

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