Resumo

RAMIREZ, Carolina; OLARTE, Ermel; TELLEZ, Eliana  e  PALMA, Marien. ANALYTICAL METHOD VALIDATION FOR DETERMINATION OF TOTAL MERCURY IN HUMAN BLOOD BY Zeeman MERCURY SPECTROMETER RA-915+ WITH THE PYROLYSIS MODULE PYRO-915+. Rev.Colomb.Quim. [online]. 2013, vol.42, n.3, pp.326-343. ISSN 0120-2804.

This article describes the methodology of the validation about the identification of total mercury in blood samples by Zeeman atomic absorption spectrophotometry RA-915+ with the module of Pyrolysis PYRO-915+, suitable for the direct determination of total mercury concentrations in various matrices, that unlike other atomization techniques (graphite furnace, cold vapor or hydride generator) does not require a sample pre-treatment. With the application of this validation,is provided the reliability in the results. Most of the validation parameters were subjected to analysis of variance ANOVA with a confidence level of 95%. It was obtained a detection limit of 2.16 üg/L, a quantitation limit of 5.54üg/L and linearity in the range of 5.54 üg/L to , in addition, the precision and accuracy was evaluated, obtaining favorable results in the analysis. The calculation of the expanded uncertainty for this method was 0.610 üg/L.

Palavras-chave : mercury; analytical methods validation; atomic absorption spectrophotometry; 100 üg/L.

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