Abstract

ARRAZOLA P., Guillermo; GRANE, Nuria; MARTIN, María L.  and  DICENTA, Federico. DETERMINATION OF CYANOGENIC COMPOUND AMYGDALIN AND PRUNASIN IN ALMOND KERNELS (PRUNUS DULCIS L) BY USING LIQUID CHROMATOGRAPHY. Rev.Colomb.Quim. [online]. 2013, vol.42, n.3, pp.365-386. ISSN 0120-2804.

The aim of this study was to apply a technique to identify and quantify separately also cyanogenic compounds that may be present in the mature seed almond (Prunus dulcis). Among the methods selected the chromatography of liquids of high resolution (HPLC), that permit the quantification of the glycosides for the separation process of  Freeze Dry where there is less surface there is more contact to sublimation without fat samples, looking at the obtain results and supported by other investigations, the use of 100 % methanol extract as a mobile phase acetonitrile-water (80:20) the results obtained of the glycosides cyanogenics resulting in a concentration of maximum amygdalin from the time of extraction of twelve hours, amygdalin is obtained, with a concentration of 9,8 mg / 100 g of dry sample. The chromatograms obtained a time of retention (Tr), amygdalin 3,4 and prunasin 5,7 two peaks with excellent resolution, to the above conditions can be used for analysis by HPLC, identification and quantification of amygdalin and prunasina.

Keywords : Cyanogens; sweet almond; amygdalin and prunasin.

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