Abstract

OCAMPO, Diana M; VALVERDE, Claudia L; COLMENARES, Ana J  and  ISAZA, José H. Total phenolics and antioxidant activity in leaves of two colombian species of Meriania genus (melastomataceae). Rev.Colomb.Quim. [online]. 2014, vol.43, n.2, pp.41-46. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v43n2.53124.

The leaves of two species, Meriania speciosa and Meriania nobilis were studied, obtaining extracts of different polarity, which were submitted to tests for their DPPH antioxidant activity (FRS₅₀: free radical scavenging capacity to reduce in 50% the DPPH) and total phenolics in a 96 wells format. This study determined that the two species have good antioxidant activity. The extracts that showed higher antioxidant activity were butanolic FRS₅₀ of 7.6 ± 0.8 and 23,4 ± 2.4; aqueous FRS₅₀ of 28.5 ± 2.9 y 63.0 ± 2.6; and hexane 2 FRS₅₀ 17.0 ± 2.6 and 18.2 ± 2.5 mg/L, for M. speciosa and M. nobilis, respectively; the extracts that showed a higher content of total phenolics were methanol 2 with a value of 0.47 ± 0.06 and 0.40 ± 0.03 and aqueous extract with a value of 0.16 ± 0.06 and 0.12 ± 0.03 mg EqAG/g ES for M. speciosa and M. nobilis, respectively. The extract showing better activity between the two species, was the butanolic of M. speciosa, with FRS₅₀ 7.6 mg/L ± 0.8, this value is comparable with the positive control, quercetine FRS₅₀: 4.2 mg/L ± 0.4, which indicates that the butanolic extract is promissory in the content of secondary metabolites with antioxidant activity and for the production of agrochemicals, cosmetics and pharmaceuticals.

Keywords : antioxidant activity; total phenolics; Melastomataceae; Meriania nobilis; Meriania especiosa.

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