Abstract

BEDOYA P, Santiago; GARCIA R, Alejandro; LONDONO F, Ángela L  and  RESTREPO C, Beatriz. Determination of organochlorine pesticide residues In serum of coffee and banana growers In the departament of quindío by gc-µecd. Rev.Colomb.Quim. [online]. 2014, vol.43, n.3, pp.11-16. ISSN 0120-2804.

Lack of knowledge of the harmful potential of pesticides by agricultural workers can cause serious health problems for them and the consumer. The residual organochlorine pesticides (OCP) was determined in serum samples of coffee and banana crops farmworkers in the department of Quindio, Colombia. To extract analytes a fast, efficient, safe, and economic assisted dispersive liquid liquid microextraction (DLLME) method was implemented by sonication, achieving extraction of 20 OCP in 200 µL of blood serum in a time of 35 min with percentage recoveries higher than 87 %. OCP were analysed by gas chromatography with electron capture detector (GC-µECD). For each analyte, the correlation coefficients obtained from internal calibration curves were higher than 0.9996, the limits of detection (LOD) were established between 0.02 to 0.04 ng/mL and the limits of quantification (LOQ) below 0.14 ng/mL. A total of 55 % of the samples analyzed (166) showed the presence of 4,4-DDE, alpha and gamma BHC, endrin aldehyde, endosulfan II, endosulfan sulfate, heptachlor, and methoxychlor, compounds that are prohibited by both national and international regulation.

Keywords : pesticides; organochlorines; lipid-solubility; serum; residues; gas chromatography.

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