Abstract

AL-HAKEIM, Hussein Kadhem; JASEM, Khlowd Mohammed  and  MOUSTAFA, Shatha Rouf. Anti-pepsin activity of silicon dioxide nanoparticles. Rev.Colomb.Quim. [online]. 2016, vol.45, n.3, pp.5-11. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v45n3.58760.

SiO₂NPs as an inhibitor of pepsin enzyme for treatment of gastro-esophageal reflux disease (GERD) were investigated. Silicon dioxide nanoparticles (pepsin coated SiO₂NPs) are among the safest nanoparticles that can be used inside the human body. The activity of pepsin before and after the addition of certain amounts of the NPs to the reaction mixture was measured spectrophotometrically. Furthermore, these experiments were repeated at different temperatures, different weights of NPs, and different ionic strengths. The kinetic parameters (K_m & V_max) of the pepsin-catalyzed reactions were calculated from the Lineweaver-Burk plots. The results showed that there is a significant reduction of pepsin activity by SiO₂NPs (V_max of free pepsin = 4.82 U and V_max of the immobilized pepsin = 2.90 U). The results also indicated that the presence of ionic strength causes remarkable reduction of pepsin activity. It can be concluded the best condition for inhibition of pepsin activity is by using a combinationof SiO₂NPs and high concentration NaCl at 37 °C.

Keywords : GERD; SiO₂nanoparticles; pepsin; enzyme inhibition.

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