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Revista Colombiana de Química
Print version ISSN 0120-2804
Abstract
CALDERON-RODRIGUEZ, Sandra Isabel and UMANA-PEREZ, Adriana. 2DE-DIGE proteomic study in blood plasma of patients in childhood stage with acute lymphoblastic leukemia. Rev.Colomb.Quim. [online]. 2019, vol.48, n.1, pp.5-15. ISSN 0120-2804. https://doi.org/10.15446/rev.colomb.quim.v48n1.75170.
In Colombia, during the last decade, acute lymphoblastic leukemia (ALL) has caused more than 40% of cancer deaths in children. Among the factors that influence these figures, late diagnosis is one of the factors that affects the treatment success. Therefore, this research focused on the plasma proteome study of Colombian children diagnosed with B-cell ALL, as compared with healthy controls in the search of proteins that could be classified as diagnostic biomarkers. Now, in view of the advances in the proteomics and mass spectrometry tools and taking into account that they are an alternative to address the molecular complexity of diseases such as cancer, a proteomic approach, based on bidimensional difference gel electrophoretic separation (2DE-DIGE) coupled to LC-MS/ MS, was used. We found eight differentially expressed proteins in plasma from B-cell ALL patients as follows: Serotransferrin, Alpha-1-antitrypsin, Haptoglobin, Zinc-alpha-2-glycoprotein, and Complement C3.
Keywords : acute lymphoblastic leukemia; two-dimensional electrophoresis; comparative proteomics; LC-MS/MS; acute phase proteins; biomarkers.