Resumo

RAMIEZ-FORERO, Gloria Smith et al. Changes in the protein profile of E. coli O157: H7 against treatment with Ib-M1 and IONP@Ib-M1. Rev.Colomb.Quim. [online]. 2021, vol.50, n.1, pp.3-12.  Epub 08-Abr-2021. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v50n1.89105.

Escherichia coli 0157: H7 is a pathogenic bacterium which is recognized for the ability to resist multiple antibiotics; accordingly, complications occur in the treatment of infections caused by this bacterium. The Ib-M1 peptide and the I0NP @ Ib-M1 bioconjugate have emerged as a new antimicrobial alternatives against E. coli 0157: H7. The mechanism of action of Ib-M1 and I0NP @ Ib-M1 against this bacterium is still unknown; therefore, the goal of this research was to identify the change in the proteins profile of E. coli 0157: H7 after treatment with Ib-M1 and I0NP @ Ib-M1 as a first step to determine its mechanism of action. For this, the proteins were obtained first, and then a two-dimensional electrophoresis was performed to finally determine the variability of the protein profiles. 0nce the protein profiles were obtained, an analysis of variance (AN0VA) was carried out. 72 differentially expressed proteins were identified, which can be connected to the effect on the bacterium's growth in the presence of Ib-M1 and I0NP @ Ib-M. These proteins are involved in acyl groups transfer processes (Yhbs protein), lipoprotein translocation (LolA protein) and amino acid transport (GpmA protein), among others.

Palavras-chave : Escherichia coli O157: H7; proteomic analysis; antimicrobial peptides; Ib-M1.

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