Abstract

SUAREZ JURADO, Aravy Ceohanna; ALVARADO, Magda Elvira; DIAZ GONZALEZ, Gonzalo Jair  and  RAMIREZ HERNANDEZ, María Helena. Evaluation and characterization of Giardia duodenalis sirtuin 2.3 (CdSir2.3). Rev.Colomb.Quim. [online]. 2022, vol.51, n.2, pp.3-10.  Epub Jan 12, 2024. ISSN 0120-2804.  https://doi.org/10.15446/rev.colomb.quim.v51n2.95495.

Giardiasis is the gastrointestinal disease with the highest incidence worldwide, caused by the protozoan Giardia duodenalis, for which there is no vaccine or efficient treatment. In order to find new pharmacological targets against this parasite, energy metabolism enzymes such as sirtuins, deacetylases dependent on the nicotinamide adenine dinucleotide (NAD), have been studied. GdSir2.1 and GdSir2.2 were previously identified as NAD-dependent deacetylases, with different subcellular locations.

In this work, another candidate for sirtuin (GdSir2.3) was studied using bioinformatic tools for the identification of typical characteristics of the sirtuin family in the sequence of the candidate; and experimental ones such as obtaining the recombinant protein 6xHis-GdSir2.3 that demonstrated NAD-dependent deacetylase activity; and that it served as an antigen in the production of IgY - α - 6xHis-GdSir2.3 for the subcellular localization of the endogenous protein in G. duodenalis. The foregoing is consistent with other studies where GdSir2.3 is indicated as an important regulator of encyst due to its increased expression during this stage of the life cycle, constituting it as a promising drug target for the control of this parasitaemia.

Keywords : Giardia; NAD; histone deacetylases; immunofluorescence; protozoa; sirtuins.

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