Abstract

NARANJO,, Julián; POSSO, Andrés; CARDENAS, Heiber  and  MUNOZ F, Jaime E.. Detección de variantes alélicas de la kappa-caseína en bovinos Hartón del Valle. Acta Agron. [online]. 2007, vol.56, n.1, pp.43-48. ISSN 0120-2812.

DNA from 2 males and 34 females of Hartón del Valle bovine breed, to detect polymorphisms by PCR-RFLP (polymerase chain reaction - restriction fragments length polymorphism) and PCR-SSCP (polymerase chain reaction- single strand conformation polymorphism) in kappa-casein gene was used 17 homozygote AA, 15 heterozygote AB and 4 homozygote BB individuals were identified. The quick and easy identification of young male genotypic carriers in less than eight hours is an advantage to milk farmers, given to there the possibility to increase the B allelic frequency, one of the ways to improve the milk efficiency for cheese producction.

Keywords : Bos Taurus; Kappa-casein; PCR-SSCP; PCR-RFLP.

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