Abstract

ARTIGAS, Rody et al. Heterochromatin decondesation in Creole cattle carrier of Robertsonian franslocation (rob 1; 29). Action of 5-azacytidine-C. Acta Agron. [online]. 2008, vol.57, n.1, pp.65-69. ISSN 0120-2812.

The Robertsonian translocation (rob1; 29) is widely spread in commercial breeds and specially in genetic reserve of American Creole cattle. It is also described a delay on embryo development in front of normal ones. The action of 5-aza-C, as an hypomethylated agent, could permitted to decondensate the constitutive or facultative heterochromatin. In this work we made induction with the 5-aza-C(10mM, 2 hrs) analogs, in lymphocyte cultures of female and male carriers and normal Creole cattle. The DNA hypometilation is found in the inactive X chromosome of late replication as it is incorporated during the last hours of cell culture. The decondensing effects of 5-aza-C analogs is observed in multiple regions of the autosomes chromatin, the rob1; 29 and the BTA1. A correlation between hypometilated regions and decondensed of facultative (conditional) heterochromatin is related with genomic instability, and epigenetic reprogramming.

Keywords : 5-Azacytidine-C; decondensation; rob1; 29; Creole cattle.

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