Resumo

PIEDRAHITA, Ana M; POSSO, Andrés; MUNOZ, Jaime E  e  ALVAREZ, Luz A. Genetic variability of Harton del Valle by RAM. Acta Agron. [online]. 2008, vol.57, n.1, pp.71-76. ISSN 0120-2812.

Harton del Valle (HV) breed belong to the seven Creole breeds of the Colombian bovine livestock, according to FAO criteria it is considered as vulnerable. To study its genetical variability, 33 HV animals and three and 3 animals of Holstein breed as a external control were sampled. Simples of DNA were isolated using the Salting Out method and RAM (Random amplified microsatellites) technique was used. CGA, CCA, TG and CT primers were used. The mean value of expected heterocigozity was 0.26 and F_ST fue 0.39 ± 0.03. Using Dice-Nei Li index and UPGMA clustering method, two groups were distinguished: the first one integrated for two conservation herds and other one by tour faros that have been sharing breeding bulls.

Palavras-chave : Genetic diversity; Creole cattle; RAM; Harton del Valle.

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