Print version ISSN 0120-2812
MARTINEZ A, Ana Karine; MORALES G, Francisco José and VALLEJO CABRERA, Franco Alirio. Molecular characterization of a begomovirus affecting tomato in the Cauca Valle - Colombia and identification of sources of resistance to improve the variety Unapal Maravilla. Acta Agron. [online]. 2008, vol.57, n.3, pp. 167-173. ISSN 0120-2812.
A virus transmitted by the whitefly Bemisia tabaci to tomato was characterized in the Cauca Valley like a variant of Tomato yellow mosaic virus (ToYMV). Artificial whitefly-mediated inoculation in the greenhouse was done with 20 days-old tomato plants (FLA 496-11-6-1-0, FLA 478-6-3-1-11, FLA 456-4 y FLA 653-3-1-0) exposed to 10 viruliferous individuals of B. tabaci (biotype B) per plant in individual insect-proof cages. The presence of the begomovirus was evaluated by symptoms development and was confirmed using dot blot hybridization and PCR. Agronomical characteristics were evaluated in the field in a completely randomized blocks design with 3 replications. The lines FLA 653-3-1-0, FLA 496-11-6-1-0 and FLA 478-6-3-1-11 developed mild symptoms, viral DNA was barely detectable in some individuals, and they showed characteristics of the fruit and desirable yield.
Keywords : Solanum lycopersicum; Bemisia tabaci; ToYMV.