Abstract

MARIN COLORADO, Jaime Alberto; RAMIREZ, Hernando  and  FREGENE, Martin. Genetic mapping and QTL analysis for carotenes in a s₁ population of cassava. Acta Agron. [online]. 2009, vol.58, n.1, pp.15-21. ISSN 0120-2812.

The S1 population of the Thai variety MTAi8 (AM320), which shows patterns of segregation defined by the content of total carotenes (beta-carotene), underwent an analysis of segregating bulk (BSA = Bulk segregating Analysis) using 700 molecular markers type microsatellites or SSR. There were generated 25 tying groups and identifying 3 major QTLs associated with a region of the genome of cassava with the content of total carotenes. Three markers SSR; explained the 37.2% (NS109), 32% (rSSRY251) and 27.7% (rSSRY313) of the total phenotypic variance, placed in the Group D strongly tying, associated with the content of total carotenes in the family AM320 S1, which established a strong positive correlation between color and content root pulp carotenes total (r = 0.81), and between negative and carotenes total dry matter (r = -0.31). The three possible QTLs showed positive and additive effect for the content of totals carotenes and could be an opportunity to implement assisted selection markers for total carotenes in cassava.

Keywords : Manihot esculenta Crantz; carotene; QTLs; SSR-microsatellites.

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