Abstract

RAMIREZ, Hernando; LENTINI, Zaida  and  VALLEJO CABRERA, Franco Alirio. Evaluation and selection of a protocol for Agrobacterium-mediated genetic transformation of tomato variety Unapal-Arreboles for resistance to budworm. Acta Agron. [online]. 2009, vol.58, n.2, pp.61-68. ISSN 0120-2812.

A plant transformation methodology was selected and evaluated to incorporate the cry1Ab gene by Agrobacterium-mediate genetic transformation into tomato variety UNAPAL-Arreboles for resistance to budworm (Tuta absoluta). A total of 59 transgenic plants were regenerated from 3.200 explants (1.84%). Histochemical gus assay and molecular analysis of three independent events To28, To33 and To47 and corresponding T1 derived generations, demonstrate the stable integration, expression and inheritance of the nptII and gus-intron genes. However, the molecular and immunological analysis of these same clones, indicate that the cry1Ab gene is not present in the transformed plants, suggesting that the sequence of this gene may be modified as result of possible recombinant events.

Keywords : Solanum lycopersicon; plant genetic transformation; Agrobacterium; Bacillus thuringiensis; budworm; Tuta absoluta.

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