Abstract

TORRES, Carlos Patiño. In vitro production of pectinases by Colletotrichum acutatum. Acta Agron. [online]. 2010, vol.59, n.1, pp.80-90. ISSN 0120-2812.

The fungi of gender Colletotrichum are producers of phytotoxic substances of low molecular weight and enzymes, which can to play crucial roles in their pathogenic relations with many commercial crops. This study was realized for to determine the type of the substances produced by C. acutatum in the Czapeck-Dox’s, Fries’s and MS’s culture medium, substances that have roles in the pathogenic relations with tamarillo (Solanum betaceae). The procedures and results of the evaluation allowed us to discard that phytotoxic activity on tamarillo fruits inoculated with extracts to be low molecular weight metabolites. Utilizing the cup-plate asay (Dingle et al., 1953), we to establish what in vitro, C. acutatum produced enzymes like pectinases (poligalacturonases) and pectyn lyases, whose action can to be implied in the antracnosis of tamarillo. Besides, the production of pectinases was infl uenced by pH’s culture medium.

Keywords : Tree tomato; Solanum betaceae; anthracnosis; Colletotrichum acutatum; pectinase; metabolites.

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