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Acta Agronómica

Print version ISSN 0120-2812

Abstract

HERNANDEZ, Erika Andrea; CAMPOS GAONA, Rómulo  and  GIRALDO PATINO, Leonidas. Metabolic behaviour in the peripartum period of dairy cows Hartón del Valle creole breed, under tropical conditions. Acta Agron. [online]. 2011, vol.60, n.1, pp.12-26. ISSN 0120-2812.

The peripartum period or transition period, presents significant physiological changes that affect production in subsequent production events (peak lactation, ovarian resumption). Creole bovine cattle "Hartón del Valle" has an excellent reproductive performance (short iterpartum period); this factor could have been originated from metabolic processes related to an improved homeostatic adjustment. The main aim of this study was to assess the homeostasis in a native cattle breed in the postpartum period. For this study, 10 multiparous cows were used and sampled before birth (days 30 and 15 prepartum) and daily in the first three days of birth, and afterwards from day 5, at intervals of five days and up to-day 60 postpartum. In total, for each animal 14 samples were analyzed which correspond to one analysis period. Using the coccygeal venipuncture technic, in vacuum tubes with and without anticoagulant the complete sample was collected. By centrifugation, plasma and serum were obtained and stored at -20°C until the metabolic analysis was done. Using a radioimmunoassay (RIA) of solid phase, hormone analysis were done. Through enzymatic colorimeter test in automated equipment the values of the metabolites for each of the defined period were determined. The mean values were: BHB (β-Hidroxibutirates) 0.39 mmol/L, NEFA (Non Esterified Fatid Acids) 0.76 mmol/L, triglycerides 0.42 mmol/L, cholesterol 2.43 mmol/L, triiodothyronine (T3) 2.69 nmol/L, tetraiodothyronine (T4) 57.37 nmol/L, insulin 4.77 mµl/ml, progesterone 5.54 nmol/L, cortisol 32.42 nmol/L. The values obtained for the different metabolites were within the biological ranges reported in the literature for cattle of tropical ecosystems.

Keywords : Adaptation; creole cattle; metabolic profile; transition period.

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