Resumo

POSSO TERRANOVA, Andrés Mauricio et al. Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites). Acta Agron. [online]. 2011, vol.60, n.2, pp.120-131. ISSN 0120-2812.

To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.

Palavras-chave : DNA; DNA isolation; genetic markers; germplasm banks; nacedero; Trichanthera gigantea.

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