Abstract

ACOSTA RANGEL, Aleyda Maritza; PENA SALAMANCA, Enrique Javier  and  CASTRO RESTREPO, Dagoberto. Evaluation of culture media for in vitro production of Annona muricata using the serial grafting technique. Acta Agron. [online]. 2011, vol.60, n.2, pp.140-146. ISSN 0120-2812.

Annona muricata is grown due to the nutritional and organoleptic quality of the fruit. The recalcitrance of the vegetative buds of this species has hindered the micropropagation of selected material according to its high productivity. This research aimed to evaluate culture medium combined with the technique of serial micrografting to promote in vitro plant growth of Annona muricata. In specific culture media for each plant material, seeds and cuttings were planted for the formation of micrografts. Three micrografting treatments were applied (M1, M2, M3) and a control, where the number indicates the times when a shoot is micrografted onto a rootstock. M1 is a monophasic micrografting or cuttings micrografted in one rootstock. M2 are micrografted cuttings for two times, that is, these remain on two different rootstocks with a lapse of one month between each transfer. M3 are micrografted cuttings three times, that is, these remain in three different rootstocks with a lapse of one month between each transfer. The shoots were separated from the rootstock and planted in culture medium to assess their reinvigoration, ANOVA and Freedman tests were performed for parametric and nonparametric data respectively. The results indicated that the monophasic micrografting is sufficient to stimulate the in vitro development of adult material of Annona muricata; however, none of the treatments was able to develop a root system in the shoots.

Keywords : Annona muricata; graft,; graft compatibility; in vitro regeneration; plant propagation; reinvigoration; soursop.

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