Abstract

PEREZ CORDERO, Alexander; ROJAS SIERRA, Johanna; CHAMORRO ANAYA, Leonardo  and  PEREZ PALENCIA, Katy. In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp. Acta Agron. [online]. 2011, vol.60, n.2, pp.158-164. ISSN 0120-2812.

The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata) in the department of Sucre, Colombia. To obtain the ethanol extract from leaves of M. officinalis and O. vulgare methods of percolation were used and for the rest of plant species, the Soxhlet method was performed. Once leaf extracts were prepared, evaluation for inhibitory activity on six isolated fungus of the genus Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 and BVC 507) was carried out. Inhibitory to the test method was used for direct seeding on the surface of potato-dextrose agar. On the different strains were added 250 µl of each extract separately. We used a positive control with nystatin (4 mg/ml) and an absolute control without any treatment. The test was evaluated by measuring the radial growth of each strain with different treatments at 96 and 168 h. A randomized complete block with three replications, to test significant differences between treatments, strains and inhibit time was used. The results expressed as percentage of inhibition, showed high activity of extracts obtained from leaves of plants of M. azederach and M. concinna, the fungi evaluated and its action was similar to the positive control.

Keywords : Anthracnose; Colletotrichum; pest control; plant extracts.

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