Resumo

PEREZ-BERNAL, Maylin; LORENZO SALINAS, Daylenis  e  DELGADO RIGO, Magalis. Viability in rice seeds obtained from plants developed in vitro. Acta Agron. [online]. 2013, vol.62, n.2, pp.114-119. ISSN 0120-2812.

The viability of rice seeds (cv. IACuba-28) obtained from in vitro developed plants was evaluated. These seeds were stored for 0, 2, 5, 7, 9 and 12 years in a seed bank at 4°C in dry and 34% relative humidity. For monitoring viability we conducted a germination test and an alpha-amylase activity assay in germinating seeds. In both experiments we set a positive control with new seeds obtained from plants grown in the field. It was observed a gradual decrease in germination percentage with increasing storage time. In the accession with 12 years of conservation the largest number of dead and contaminated seeds and abnormal plants was found. Accessions showed increasing in alpha amylase activity until the fifth day of germination and then decreased, but quantitative significant differences (P<0.05) among young and old accessions were found. The average of alpha amylase activity on the fifth day was 0.25 for young accessions, while for the oldest was 0.192. The highest difference was noted in the accession of 12 years, where the maximum activity only reached 0.0614. The present study demonstrated that seeds from in vitro culture maintain their viability for 10 years of storage. After this time, we perceived adverse changes in germination parameters and in the alpha amylase activity.

Palavras-chave : Alpha amylase activity; germination test; Oryza sativa.

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