Abstract

LONDONO-GIRALDO, Lina María  and  GUTIERREZ-LOPEZ, Luis Gonzaga. Regeneration of coffee-shading Walnut (Cordia alliodora (Ruiz and Pav.) Oken) from indirect organogenesis. Acta Agron. [online]. 2013, vol.62, n.3, pp.207-214. ISSN 0120-2812.

This study, using indirect organogenesis from cotyledonal leaf evaluated Cordia alliodora (Ruiz y Pav) Oken regeneration, a tree native to Colombia with industrial, medical and aesthetic properties. The seeds were first treated with 3% sodium hypochlorite solution during 15 minutes supplemented with PPM® added to the culture medium to remove any bacteria and fungi. Three hormones were tested in the induction phase: BAP, Picloram and 2,4-D in concentrations from 0,5 to 12,5 mg/L in light and dark conditions; production of callus was obtained in all of them. After thirty days most of the callus were oxidized; some callus on BAP 10mg/L showed organogenic shoots. These results suggest a way for in vitro regeneration through indirect organogenesis from coffee-shading walnut cotyledonal leaves and suggest that more research proceed in evaluating this regeneration technique and other in vitro regeneration techniques.

Keywords : native forest.

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