Abstract

LEGUIZAMO, María Claudia et al. Control of Fusarium spp and Bacillus subtilis through metabolites of Xenorhabdus bovienii mutualist of Steinernema feltiae. Acta Agron. [online]. 2014, vol.63, n.1, pp.55-62. ISSN 0120-2812.  https://doi.org/10.15446/acag.v63n1.40557.

The antimicrobial activity of metabolites of Xenorhabdus bovienii (Enterobacteriaceae) was evaluated, mutualist of the entomoparasite nematode Steinernema feltiae (Filipjev, 1934 Cephalobina, Steinernematidae) over Fusarium spp. and B. subtilis. X.bovieniiwas purified from Galleria mellonella larvae infected with S. feltiae and extracted in a bioreactor. Its growth was sampled at intervals of 4h for 96h of culture, a time on which metabolites were extracted by using six methods: organic compounds, proteins, Indole compounds, methanol, column chromatography and butanol. The metabolites extracted from X.bovienii at four concentrations were faced with B. subtilis and Fusarium spp. in tomato seeds, Santa Clara's variety. The inhibition halo (mm) was assessed for B. subtillis and for Fusarium in the presence of macro, microconidia, and chlamydospores. Phytotoxicity was assessed X. bovienii showed higher growth and the presence of metabolites in the first 16-20 h of incubation up to 40h. The maximum inhibition occurred at concentrations of 100% and early sampling. In Fusarium spp. mycelial growth inhibition coincided with a lower presence of macro, microconidia, and chlamydospores. No phytotoxic effects of the metabolites tested were observed. The potential of X. bovienii was tested for control of Fusarium spp. and B. subtilis as a microorganism sensitive to changes by these metabolites.

Keywords : Bacillus subtillis; biological control; Fusarium spp; secondary metabolites; Xenorhabdus bovienii.

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