Abstract

CEBALLOS, Germán; ALVAREZ, Elizabeth  and  BOLANOS, Martha M. Reduction in populations of Ralstonia solanacearum race 2 in plantain (Musa AAB Simmonds) with extracts from Trichoderma sp. and antagonistic bacteria,. Acta Agron. [online]. 2014, vol.63, n.1, pp.80-87. ISSN 0120-2812.  https://doi.org/10.15446/acag.v63n1.43121.

Moko, bacterial disease caused by Ralstonia solanacearum race 2, affects plantain production in Colombia, with losses up to 100 %. The in vitro effect of crude filtrates of strains of Trichoderma spp. and two commercial products were evaluated versus R. solanacearum. In vitro, filtrate was used (40 % in aqueous solution) for each strain of Trichoderma spp.; from R. solanacearum a dilution of 10^-4 per petri dish was used, in a completely random design with three replications. For Ralstonia solanacearum, the number of colony forming units was determined. In greenhouse, the suspensions of Trichoderma harzianum (Agroguard ®), T. viride 14PDA3A, and Ecoterra® were applied to Dominico harton plantain plants, and 30 days later plants were inoculated with R. solanacearum, a randomized complete blocks design with four blocks was used. R. solanacearum severity was evaluated by leaf symptoms scale, of 0 to 6. In vitro, crude extracts of two strains and two commercial products of Trichoderma spp. inhibited 100% of R. solanacearum. T. viride and Ecoterra® treatments showed low levels of disease severity by R. solanacearum in plants (0.63 and 1.88 respectively).

Keywords : Antagonism; Dominico Harton plantain; moko disease; Trichoderma spp.

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