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Acta Agronómica

Print version ISSN 0120-2812

Abstract

CABRERA NAVARRO, Sergio Andrés; SANDOVAL ALDANA, Angélica Piedad  and  FORERO LONGAS, Freddy. Antioxidant and antimicrobial potential of aqueous and hidroalcoholic extracts of granadilla (Passiflora ligularis). Acta Agron. [online]. 2014, vol.63, n.3, pp.204-211. ISSN 0120-2812.  https://doi.org/10.15446/acag.v63n3.41976.

The main objective of this study was to determine the antioxidant and antimicrobial activity in aqueous and hydroalcoholic extracts of Passiflora ligularis. It was used the reflux extraction method, using as solvent water, ethanol 35% (v/v) and 70% (v/v), working with leaves and flowers as plant material. The analysis demonstrated that both aqueous and hydroalcoholic extracts present phenolic compounds, achieving maximum levels of 14.32 mg Eq Ac. Gal /g dry matter. Also was determined maximum total flavonoids equivalent to 10.47 mg Eq Vitexin/g dry matter on hydroalcoholic extracts. in vitro antioxidant activity of the extracts was evaluated using the methodology of capturing the free radical (DPPH) and Ferric Reducing Power (FRAP), in both methods was determined that hydroalcoholic extracts were more active. The antimicrobial activity test, indicated that the extracts of P. ligularis have the ability to reduce the growth of both E. coli (ATCC 25922) and S. aureus (ATCC 25923), mainly finding that aqueous extracts possess greater microbial growth inhibition than the hydroalcoholic ones. In this study also was possible to identify a correlation between the phenols and antioxidant activity (FRAP).

Keywords : Reflux extraction; flavonoids; phenols; free radicals.

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