Abstract

MARTINEZ PACHECO, Leonardo et al. PCR detection of Colletotrichum lindemuthianum in seeds and crops of common bean from Antioquia, Colombia. Acta Agron. [online]. 2014, vol.63, n.4, pp.377-387. ISSN 0120-2812.  https://doi.org/10.15446/acag.v63n4.42035.

Colletotrichum lindemuthianum, the causal agent of anthracnose in common bean, is one of the most important pathogens of this crop around the world. Accurate detection and identification of this fungus is of paramount importance for adequate management of this disease; in this respect, molecular tests are the fastest and most sensitive methods. In this work, the usefulness of four primer sets (CY1/CY2, CD1/CD2, ClF4/ITS4 and ClF432/ClR533) for PCR detection and identification of C. lindemuthianum was evaluated using leaf, pod and seed tissues of common bean crops from Antioquia, Colombia. The results suggest that primers CD1/CD2, targeting iron permease pseudogen Ftr1, were the most effective in detecting DNA from both microbiological isolates and infected bean tissues, including seeds. In the case of primers CY1/CY2 targeting the rDNA ITS region, a PCR-RFLP strategy is recommended using MseI (=Tru1I) to differentiate C. lindemuthianum from C. orbiculare and C. trifolii. These primers produced better results when combined with universal primers ITS1 (ITS1/CY2) and ITS4 (CY1/ITS4). Primers ClF4/ ITS4 were non-specific while ClF432/ClR533 result in bands with poor resolution in agarose gels. This study will serve as support of seed certification and genetic improvement programs in common bean.

Keywords : Anthracnose; Iron permease; PCR; Phaseolus vulgaris; RFLPs; Ribosomal DNA.

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