Abstract

REVELO PORTILLA, Ediel Armando; CARDOZO CONDE, Carlos Iván  and  CAETANO, Creucí María. A molecular preliminary study of Colombian maize (Zea mays L.) accessions by using a cpDNA region. Acta Agron. [online]. 2015, vol.64, n.1, pp.72-82. ISSN 0120-2812.  https://doi.org/10.15446/acag.v64n1.40724.

In order to preliminarily explore the genetic diversity in the 23 Colombian races of maize described by Roberts and co-workers (1957), this study evaluate 28 nuclear and chloroplast primers. Of these, 14 amplified in the PCR, which were sent to sequencing to Macrogen Inc. (Korea). Bioinformatics programs revealed that six of these primers had a high level of polymorphism. It was considered that the genomic chloroplast region atpB-rbcL-1-1 showed the highest polymorphism, therefore was used to evaluate 23 materials, representative of the 23 races, stored in the CIMMYT germplasm bank, Mexico. By analyzing sequences we could defend and confront racial groups obtained in the present study ('primitive races' three, 'probably introduced' seven, 'Colombian hybrid', 13) with those established by Roberts and co-workers at 50, shown two 'primitive', nine 'probably introduced' and twelve 'Colombian hybrid' and the groups set by Cardona (2010), applying the strategy Ward-MLM for the same characters described by Roberts et al. (1957), which shows five 'primitive', seven 'probably introduced' and nine 'Colombian hybrid' races. Thus, it makes a methodological contribution to validate historical data and redefine racial groups

Keywords : cpDNA; genetic diversity; Zea mays L; maíz criollo; razas criollas; Colombia.

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