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Acta Agronómica

Print version ISSN 0120-2812

Abstract

MARTIN, Thomas Newton; FIPKE, Glauber Mongon; MINUSSI WINCK, José Eduardo  and  MARCHESE, José Abramo. ImageJ software as an alternative method for estimating leaf area in oats. Acta Agron. [online]. 2020, vol.69, n.3, pp.162-169.  Epub Aug 24, 2021. ISSN 0120-2812.  https://doi.org/10.15446/acag.v69n3.69401.

The aim of this study was to compare two methods of estimating leaf area (leaf area meter LI-COR 3100 and ImageJ software) in oats. Renascem black oats and UTF Iguaqu white oats cultivars were evaluated. All the leaves of one plant per plot, with six repetitions, were evaluated every seven days to obtain five assessments for each cultivar. The leaves were identified and fixed on a white A4 sheet of paper with an indication of a known area (45 cm2), photographed and evaluated using a leaf area meter (LI-3100 - LI-COR). Leaf area was then estimated using ImageJ software. Estimates were obtained for standard deviation and coefficient of variation, and a simple linear regression equation was estimated based on the two estimation methods. Mean variances were tested using the ‘F’-test and the means compared by the ‘t’-test. There was no difference between the leaf areas found by both methods. In addition, they were highly correlated, and variances were homogeneous. We concluded that ImageJ software can be used instead of the leaf area meter on the two oat cultivars evaluated.

Keywords : Avena strigosa Schreb.; Avena sativa L.; leaf area meter; LI-COR 3100.

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