Resumo

CORRALES-CABRA, Eríka et al. Molecular detection of viruses in Cape gooseberry (Physalis peruviana) crops from Southwestern Antioquia (Colombia). Acta Agron. [online]. 2022, vol.71, n.1, pp.88-95.  Epub 02-Maio-2023. ISSN 0120-2812.  https://doi.org/10.15446/acag.v71n1.99453.

Cape gooseberry is one of the most promising fruit crops in the Colombian Andes because of its high demand in the international market. In the province of Antioquia, this crop is cultivated mostly in the east, but optimal conditions are also available in the northern and southwestern regions. As viral diseases have become a serious emergent threat to this crop, we have investigated the RNA virus infection in Cape gooseberry crops from southwestern Antioquia. The presence of viruses was tested by RT-qPCR of leaves of symptomatic and asymptomatic plants from eight commercial plots, and eight seed samples from commercial vendors in the region. Our results revealed the presence of PVY, PVX, PVS, CGIV-1, and PMTV in at least one sample of commercial plots; neither PVV, PYVV nor PhyVNV were detected in these samples. PMTV and PVX were the most prevalent viruses in symptomatic and asymptomatic adult plant samples with 75.0 and 62.5 %, respectively. Analysis of seeds revealed 87.5 % prevalence for PVX, 75.0 % for PVS, 37.5 % for PVY, 12.5 % for PhyVNV, and 12.5 % for CGIV-1. A next generation sequencing revealed sequence reads of CMV, PhyVNV, PVS, and TaLMV. These results highlight the need to implement surveillance programs that monitor the health status of commercial plots, and to get certified seed of P. peruviana.

Palavras-chave : seed certification; phytopathology; real time PCR; Solanaceae; plant viruses.

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