Resumo

SALAZAR-MERCADO, Seir Antonio  e  PALENCIA-DELGADO, Yordan Arley. Management of different pretreatments to optimize seed viability of orchids. Acta Agron. [online]. 2023, vol.72, n.4, pp.346-352.  Epub 28-Abr-2025. ISSN 0120-2812.  https://doi.org/10.15446/acag.v72n4.112433.

Orchids are one of the most difficult plant families to propagate, so it is important to determine the viability of their seeds. In this sense, the tetrazolium test and in vitro germination are the best alternatives to evaluate their germination capacity in a fast, accurate, and effective way. Considering the above, the aim of this research was to evaluate the viability of Pleurothallis sp, Spathoglottis plicata, and Stelis sp. seeds, using different pretreatments to optimize the tetrazolium test. For this, the efficacy of five pretreatments was compared in which the sedes were subjected to different solutions: chlorine 0.1 %, chlorine 0.2 %, deionized water (H2Od), 10 % (w/v) sucrose solution, and a control (no pretreatment), for a period of 8 minutes. Then, the seeds were submerged in two doses of tetrazolium (0.25 % and 0.5 %) during three exposure times (6, 12, and 24 h), in complete darkness. Subsequently, the basal MS culture medium was used for 55 days to evaluate in vitro germination. According to the obtained results, it was observed that the pretreatments with deionized water (H2Od) and the 10 % sucrose solution presented the best viability indexes for each of the studied species, which correlated with the in vitro germination percentages obtained. It should be emphasized that Stelis sp. was the species with the best index, with 99 % using the pretreatment of deionized water at a dose of 0.25 % and for a exposure time of 24 hours, and with 100 % viability using the pretreatment of 10 % sucrose solution at a dose of 0.25 % for 24 hours.

Palavras-chave : germination; Pleurothallis sp; Spathoglotti plicata; Stelis sp; tetrazolium.

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