Abstract

OCAMPO, R. J et al. FIRST REPORT OF Cryptosporidium parvum IN HOLSTEIN CALVES (Bos taurus) FROM MANIZALES, CALDAS, COLOMBIA. Rev. Med. Vet. Zoot. [online]. 2012, vol.59, n.3, pp.159-164. ISSN 0120-2952.

The objective of this study was to identify species or genotypes of Cryptosporidium parasite present in feces collected from Holstein calves in Manizales city, Caldas Department, Colombia. DNA was extracted from 80 fecal samples, which 11 were diagnosed positive for Cryptosporidium spp., by the Polymerase Chain Reaction method. PCR-RFLP analysis of 18S rDNA locus identified the presence of Cryptosporidium parvum in all samples tested positive. This finding suggests that cattle may be a potential source of human infection by Cryptosporidium, and it becomes the first published report of C. parvum in cattle in Manizales, Caldas.

Keywords : Cryptosporidium spp.; 18S rDNA; PCR-RFLP.

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