Resumen

POCHES, R. A; BRIEVA, C. I  y  JIMENEZ, C. SEMINAL CHARACTERISTICS OF WHITE-FOOTED TAMARIN (Saguinus leucopus) UNDER CAPTIVITY CONDITIONS OBTAINED BY PENILE VIBRATORY STIMULATION (PVS). Rev. Med. Vet. Zoot. [online]. 2013, vol.60, n.1, pp.11-22. ISSN 0120-2952.

The efficacy of the penile vibratory stimulation (PVS) for semen collection was evaluated in fifteen (15) specimens of white-footed tamarin (S. leucopus), held in captivity, at four different locations. Additionally the technique was standardized for semen collection in this species and seminal characteristics, color, viscosity, volume, pH, motility, morphology, viability, sperm concentration and morphometry were also evaluated. Ketamine hydrochloride was used at a dose between 5 to 10 mg/Kg for sedation and semen sampling. The technique was successful in 52.6% of the animals using the combination of 90 Hertz of vibration and 1 mm of amplitude; time of ejaculation was 12:3 ±6:42 minutes. Seminal characteristics were: pH of 7.5±0.26; volume of 24±18.82 µl; masal motility of 3.7/5.0±0.5; progressive motility of 97.1±45.4%; sperm concentration of 87,617±21,327 x 10⁴spz/µl; normal morphology 69.3±11.06% and viability of 93.7±4.9%. Talp-Hepes extender was satisfactorily used and it did not appear to affect seminal characteristics. The PVS is an innovating, replicable, viable and safe method for obtain semen in S. leucopus and perhaps in other callitrichids under ketamine sedation and in captivity.

Palabras clave : callitrichids; penile vibratory stimulation (PVS); semen; spermiogram; extender; captivity.

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