Abstract

RIANO, N. B  and  VERA, V. J. ISOLATION, CHARACTERIZATION AND POTENTIAL DIFFERENTIATION OF CANINE MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE. Rev. Med. Vet. Zoot. [online]. 2014, vol.61, n.2, pp.115-133. ISSN 0120-2952.  https://doi.org/10.15446/rfmvz.v61n2.44675.

Cells obtained from the canine subcutaneous adipose tissue were characterized to demonstrate the nature of mesenchymal stem cells (adMSCs). The cells were cultured and expanded in vitro conditions. The presence of the surface markers CD73, CD90 and CD105 by flow cytometry and real time PCR was determined. The expression profile is identical to that reported in human mesenchymal stem cells. The differentiation potential was demonstrated by gene expression by real time PCR after induction osteogenic and adipogenic. Osteogenic differentiation was evidenced by the expression of genes of bone tissue (type I collagen and osteonectin) in expression kinetics consistent with the molecular and cellular events of bone development in vivo. In the adipogenic differentiation, were the formation of intracellular lipid vacuoles and expression of lipoprotein lipase gene (LPS), according to the in vivo process. We conclude that mesenchymal, cells derived from adipose tissue of dog, meet the morphological criteria, expression of specific surface markers and mesodermal differentiation capacity, confirming the nature of mesenchymal stem cells. The canine mesenchymal stem cells are an excellent preclinical model for developing applications in regenerative medicine that can be used both in veterinary and human medicine.

Keywords : Mesenchymal canine stem cells; osteogenic differentiation; adipogenic differentiation.

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